Timber buildings are resistant to dynamic loads due to their low weight and relatively high rigidity. However, the connections between wooden elements are the key elements of their rigidity. This study investigates how two common fasteners—nails and screws—affect the dynamic response of wooden frames. Experimental tests have been conducted on two different wooden frames. The first one is connected with nails while the second one is connected with screws. Standard wall frame elements were subjected to harmonic excitation at various frequencies and displacement amplitudes. The research aimed to assess the impact of the typical structural fasteners used on the rigidity of the wooden frame in global terms. Experimental results indicate that replacing nails with screws significantly enhances the dynamic performance of wooden wall frames. The results show a very good and effective method of strengthening skeletal structures, which can be implemented at a low cost at the initial design and during the construction stage of the structures.